Isolation and Identification of Biofilm-Forming Staphylococcus Aureus in Commercial Cow Milk Products

Authors

  • Dominggas Yambise
  • Catarina Aprilia Ariestanti
  • Tri Yahya Budiarso

DOI:

https://doi.org/10.21460/sciscitatio.2020.12.33

Keywords:

Staphylococcus aureus, packed milk product, biofilm, API STAPH

Abstract

Food poisoning caused by the contamination from Staphylococcus aureus are frequently found in food
especially in dairy products. Pasteurization process in milk production was not enough to kill S. aureus because
it formed biofilm that could survive in high temperature. This research aimed to study the presence of biofilmforming S. aureus in samples from packed commercial milk products in Yogyakarta City, Indonesia. Twenty
isolates from dairy products were grown in Brain Heart Infusion (BHI) broth then inoculated into Braid-Parker
Agar (BPA) medium to get the candidate of S. aureus isolates. These isolate candidates were selected using
Mannitol Salt Agar (MSA) and Congo Red Agar (CRA) medium. Another selection was done by carbohydrate
fermentation analysis and confirmed using API STAPH. Confirmation analysis showed that eight isolates
were identified as S. aureus. Another two isolates were identified as S. xylosus and S. haemolyticus. Therefore,
it indicated the presence of Staphylococcus aureus as contaminant in dairy products.

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Published

2020-08-28