Deteksi Gen alg44, oprL dan toxA pada Pseudomonas aeruginosa Isolat Klinis dengan Metode Polymerase Chain Reaction
DOI:
https://doi.org/10.21460/sciscitatio.2025.62.225Keywords:
alg44, oprL, toxA, Pseudomonas aeruginosa, PCRAbstract
Gen alg44, oprL dan toxA merupakan gen yang berperan dalam faktor virulensi pada bakteri Pseudomonas aeruginosa yang dapat menyebabkan infeksi kronis pada manusia. Tujuan dari penelitian ini adalah untuk mendeteksi adanya gen alg44, oprL dan toxA pada Pseudomonas aeruginosa dengan menggunakan isolat klinis berupa sputum, urine dan pus dengan metode Polymerase Chain Reaction. Penelitian ini dimulai dengan melakukan karakterisasi dari 6 sampel isolat klinis Pseudomonas aeruginosa yang berupa sputum, urine dan pus (masing-masing 2) melalui uji mikroskopis, makroskopis dan uji biokimia. Proses isolasi DNA bakteri menggunakan Presto Mini gDNA Bacteria Kit, hasil dari isolasi DNA dilakukan uji kualitatif elektroforesis gel agarosa dan uji kuantitatif dengan Spekrofotometer UV-Vis. Deteksi gen dilakukan pada enam sampel DNA Pseudomonas aeruginosa menggunakan metode Polymerase Chain Reaction dengan primer spesifik. Hasil dari PCR menyatakan bahwa gen alg44, oprL dan toxA terdeteksi pada semua isolat Pseudomonas aeruginosa.
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